TIMELINE FOR RESEARCH (VETS)
A tentative schedule of things we will do over the first few weeks. Researcher's Names:
Summer Veterans: Aldo, Andrew, Michael, Daniel, Janice, Max, Urvashi, Alex, Kaarthik, Aakash, Sidestreamers: Rishi, Paul, Priya, Vanna, Keyur, Ben Springers:
NOTE - see bottom of page for previous weeks' schedules and things to catch up on.
This Week
Finish cloning your gene into pNIC-Bsa4
Transform positive clone with your gene into BL21(De3) cells - begin expression
PyMol Refresher - Due by ??
Science Works assignment due ??
27-Aug Monday
28-Aug Tuesday
29-Aug Wednesday
2 - 3pm - Lab Group Meeting/Journal Club (in ???? classroom)
30-Aug Thursday
31-Aug Friday
Virtual Screen Refresher - Due ??
Help Sessions: PyMol and Virtual Screen Refresher Welch Computer Lab
Wikispaces check on each Friday
1-Sep Saturday 2-Sep Sunday
Next Week
3-Sep Monday
4-Sep Tuesday
5-Sep Wednesday
2 - 3pm - Lab Group Meeting/Journal Club (in ???? classroom)
6-Sep Thursday
7-Sep Friday Lab Notebook Check this Friday- leave your notebook in 'Drug Screening Notebooks' drawer in lab by 7:00 pm
should include all current results and commentary
Wikispaces check on each Friday
8-Sep Saturday 9-Sep Sunday
Begin realVirtual Screening - prepare protein in GOLD and start jobs (cb306 should be done this week and the next one started)
Week 7 (10/10 – 10/14)
Journal Club 3
Research Report Due - Tues Oct. 11th - Guidelines in GDocs folder
Virtual Screening help session for setting up your protein (should have started your second library this week)
Monday 4 - 5:30 in WEL 2.306 Computer Lab.
Express and Purify and Characterize your protein PyMol Image contest Due Friday at Midnight
Wikispaces check on each Friday Week 8 (10/17 – 10/21) VIRTUAL SCREENING - should have 3 libraries completed, at the least
FPLC purify enzyme, store enzyme
Test your enzyme for activity
Wikispaces check on each Friday
Week 9 (10/24 – 10/28)
Journal Club 4 VIRTUAL SCREENING - finish by this week - do Top Ligands Procotol to get final compounds for ordering
FPLC purify enzyme, store enzyme
Test your enzyme for activity Fri ( 10/28) Lab Notebook check this Friday(7:00 pm)
or Sat night if working on Sat.
Wikispaces check on each Friday
Protein Expression Scheduling:
Here's the Sign Up Form on Google - new guys and returning Spring VDSers should fill this out by Monday night.
Week 10 (10/31 – 11/4) Tues Nov. 1st Thursday Nov 3rd at Midnight (Materials & Methods for Cloning due) FRI External Open House - Wednesday Nov. 2nd 1- 4 pm Top Ligands Procotol to get final compounds for ordering
FPLC purify enzyme, store enzyme
Test your enzyme for activity
Wikispaces check on each Friday Week 11 (11/7 – 11/11)
Journal Club 5
Inhibition Assays
Tues Nov. 8th at Midnight (Materials & Methods for Virtual Screeningdue) - see Tips & Hints page
Wikispaces check on each Friday Week 12 (11/14 – 11/18) Tues Nov. 15th at Midnight (Materials & Methods for Expression, Purification, and Characterization due)
Purify and Characterize your protein - NOTE: this is a long and arduous process. Don't put it off.
Inhibition Assays Fri ( 11/18) Lab Notebook check this Friday(7:00 pm)
or Sat night if working on Sat.
Wikispaces check on each Friday
Protein Protocols to be completed - also see instructional videos on GDocs
ProtocolLabEnzymeTest_Phosphatase_VDS_Fall11.xlsx
Then on to Inhibition Assays once compounds arrive Week 13 (11/21 – 11/25): Thanksgiving
Journal Club 6
Inhibition Assays - on 2 compounds
Week 14 (11/28 – 12/2) - Last Class week
Inhibition Assays - on 2 compounds
Intro to ICM?
Tues Nov. 29th - FINAL PRESENTATIONS of Enzyme Inhibition Graphs - everyone
make a one slide PowerPoint and upload to the GroupMeetingSlides/FInalSlides_StudentsFall11 folder on GDocs
save file with your name and date in the file name
Thurs (12/1) - Lab Cleanup (Required) - each students spends 1 hr cleaning in lab (counts as 1 hour of lab time) Fri (12/2) - Lab Cleanup (Required) - each students spends 1 hr cleaning in lab (counts as 1 hour of lab time)
Sat ( 12/3) Final Report due (5:00 pm ) Final Lab Notebook check (7:00 pm)
Final Wikispaces check
A tentative schedule of things we will do over the first few weeks.
Researcher's Names:
Summer Veterans: Aldo, Andrew, Michael, Daniel, Janice, Max, Urvashi, Alex, Kaarthik, Aakash,
Sidestreamers: Rishi, Paul, Priya, Vanna, Keyur, Ben
Springers:
NOTE - see bottom of page for previous weeks' schedules and things to catch up on.
This Week
Finish cloning your gene into pNIC-Bsa4Transform positive clone with your gene into BL21(De3) cells - begin expression
PyMol Refresher - Due by ??
Science Works assignment due ??
27-Aug Monday
28-Aug Tuesday
29-Aug Wednesday
2 - 3pm - Lab Group Meeting/Journal Club (in ???? classroom)
30-Aug Thursday
31-Aug Friday
Virtual Screen Refresher - Due ??
Wikispaces check on each Friday
1-Sep Saturday
2-Sep Sunday
Next Week
3-Sep Monday4-Sep Tuesday
5-Sep Wednesday
2 - 3pm - Lab Group Meeting/Journal Club (in ???? classroom)
6-Sep Thursday
7-Sep Friday
Lab Notebook Check this Friday- leave your notebook in 'Drug Screening Notebooks' drawer in lab by 7:00 pm
Wikispaces check on each Friday
8-Sep Saturday
9-Sep Sunday
Begin real Virtual Screening - prepare protein in GOLD and start jobs (cb306 should be done this week and the next one started)
10-SepMonday
11-SepTuesday
12-SepWednesday
13-SepThursday
14-SepFriday
15-SepSaturday
16-SepSunday
17-SepMonday
18-SepTuesday
19-SepWednesday
20-SepThursday
21-SepFriday
22-SepSaturday
23-SepSunday
24-SepMonday
25-SepTuesday
26-SepWednesday
27-SepThursday
28-SepFriday
29-SepSaturday
30-SepSunday
1-Oct Monday
2-Oct Tuesday
3-Oct Wednesday
4-Oct Thursday
5-Oct Friday
6-Oct Saturday
7-Oct Sunday
8-Oct Monday
9-Oct Tuesday
10-OctWednesday
11-OctThursday
12-OctFriday
13-OctSaturday
14-OctSunday
15-OctMonday
16-OctTuesday
17-OctWednesday
18-OctThursday
19-OctFriday
20-OctSaturday
21-OctSunday
22-OctMonday
23-OctTuesday
24-OctWednesday
25-OctThursday
26-OctFriday
27-OctSaturday
28-OctSunday
29-OctMonday
30-OctTuesday
31-OctWednesday
1-Nov Thursday
2-Nov Friday
3-Nov Saturday
4-Nov Sunday
5-Nov Monday
6-Nov Tuesday
7-Nov Wednesday
8-Nov Thursday
9-Nov Friday
10-NovSaturday
11-Nov Sunday
12-Nov Monday
13-Nov Tuesday
14-Nov Wednesday
15-Nov Thursday
16-Nov Friday
17-Nov Saturday
18-Nov Sunday
19-Nov Monday
20-Nov Tuesday
21-Nov Wednesday
22-Nov Thursday
23-Nov Friday
24-Nov Saturday
25-Nov Sunday
26-Nov Monday
27-Nov Tuesday
28-Nov Wednesday
29-Nov Thursday
30-Nov Friday
1-Dec Saturday
2-Dec Sunday
3-Dec Monday
4-Dec Tuesday
5-Dec Wednesday
6-Dec Thursday
7-Dec Friday
8-Dec Saturday
9-Dec Sunday
10-DecMonday
11-DecTuesday
12-DecWednesday
13-DecThursday
14-DecFriday
15-DecSaturday
16-DecSunday
17-DecMonday
18-DecTuesday
From Fall11
Week 7 (10/10 – 10/14)
Journal Club 3
Research Report Due - Tues Oct. 11th - Guidelines in GDocs folder
Virtual Screening help session for setting up your protein (should have started your second library this week)
Express and Purify and Characterize your protein
PyMol Image contest Due Friday at Midnight
Wikispaces check on each Friday
Week 8 (10/17 – 10/21)
VIRTUAL SCREENING - should have 3 libraries completed, at the least
FPLC purify enzyme, store enzyme
Test your enzyme for activity
Wikispaces check on each Friday
Week 9 (10/24 – 10/28)
Journal Club 4
VIRTUAL SCREENING - finish by this week - do Top Ligands Procotol to get final compounds for ordering
FPLC purify enzyme, store enzyme
Test your enzyme for activity
Fri ( 10/28) Lab Notebook check this Friday(7:00 pm)
or Sat night if working on Sat.
Wikispaces check on each Friday
Protein Expression Scheduling:
Here's the Sign Up Form on Google - new guys and returning Spring VDSers should fill this out by Monday night.Week 10 (10/31 – 11/4)
Tues Nov. 1st Thursday Nov 3rd at Midnight (Materials & Methods for Cloning due)
FRI External Open House - Wednesday Nov. 2nd 1- 4 pm
Top Ligands Procotol to get final compounds for ordering
FPLC purify enzyme, store enzyme
Test your enzyme for activity
Wikispaces check on each Friday
Week 11 (11/7 – 11/11)
Journal Club 5
Inhibition Assays
Tues Nov. 8th at Midnight (Materials & Methods for Virtual Screeningdue) - see Tips & Hints page
Wikispaces check on each Friday
Week 12 (11/14 – 11/18)
Tues Nov. 15th at Midnight (Materials & Methods for Expression, Purification, and Characterization due)
Purify and Characterize your protein - NOTE: this is a long and arduous process. Don't put it off.
Inhibition Assays
Fri ( 11/18) Lab Notebook check this Friday(7:00 pm)
or Sat night if working on Sat.
Wikispaces check on each Friday
Protein Protocols to be completed - also see instructional videos on GDocs
ProtocolProteinExpression_VDS_Fall11_ver2.docProtocolProteinPurificationVDS_Fall11.doc
ProtocolProteinCharacterizationVDS_Fall11.doc
ProtocolFPLC_GelFiltration_VDS_Fall11V_v2.docx
ProtocolLabEnzymeTest_Phosphatase_VDS_Fall11.xlsx
Then on to Inhibition Assays once compounds arrive
Week 13 (11/21 – 11/25): Thanksgiving
Journal Club 6
Inhibition Assays - on 2 compounds
Week 14 (11/28 – 12/2) - Last Class week
Inhibition Assays - on 2 compounds
Intro to ICM?
Tues Nov. 29th - FINAL PRESENTATIONS of Enzyme Inhibition Graphs - everyone
make a one slide PowerPoint and upload to the GroupMeetingSlides/FInalSlides_StudentsFall11 folder on GDocs
save file with your name and date in the file name
Thurs (12/1) - Lab Cleanup (Required) - each students spends 1 hr cleaning in lab (counts as 1 hour of lab time)
Fri (12/2) - Lab Cleanup (Required) - each students spends 1 hr cleaning in lab (counts as 1 hour of lab time)
Sat ( 12/3)
Final Report due (5:00 pm )
Final Lab Notebook check (7:00 pm)
Final Wikispaces check